high-affinity choline transporter (slc5a7/cht1) antibody (Thermo Fisher)
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high-affinity choline transporter (slc5a7/cht1) antibody
High Affinity Choline Transporter (Slc5a7/Cht1) Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cht1/cht1+antibody/pm37827341-89-41-46
Average 90 stars, based on 1 article reviews
High Affinity Choline Transporter (Slc5a7/Cht1) Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cht1/cht1+antibody/pm37827341-89-41-46
Average 90 stars, based on 1 article reviews
high-affinity choline transporter (slc5a7/cht1) antibody - by Bioz Stars,
2026-11
90/100 stars
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other:Article Title: Activation of the High-Affinity Choline Transporter 1 in the Spinal Cord Relieves Stress-Induced Hyperalgesia. Article Snippet: Background The mechanism underlying irritable bowel syndrome (IBS), a common disease with hyperalgesia, remains elusive.. The spinal cholinergic system is involved in pain modulation, but its role in IBS is unknown.. Aims To determine whether high-affinity choline transporter 1 (CHT1, a major determinant of the cholinergic signaling capacity), is implicated in spinal modulation of stress-induced hyperalgesia. Article Title: CDP-choline modulates cholinergic signaling and gut microbiota to alleviate DSS-induced inflammatory bowel disease. Article Snippet: The membranes were sealed (20 °C, 12 min) in protein-free rapid blocking buffer (EpiZyme, PS108P, China) and incubated overnight at 4 °C in the following primary antibody: β-Actin (1:1000, Cell Signaling Technology, #3700, USA), Occludin (1:1000, Cell Signaling Technology, #91131, USA), Article Title: Biochemical characterization of two novel mutations in the human high-affinity choline transporter 1 identified in a patient with congenital myasthenic syndrome. Article Snippet: Congenital myasthenic syndrome (CMS) is a heterogeneous condition associated with 34 different genes, including SLC5A7, which encodes the high-affinity choline transporter 1 (CHT1).. CHT1 is expressed in presynaptic neurons of the neuromuscular junction where it uses the inward sodium gradient to reuptake choline.. Biallelic CHT1 mutations often lead to neonatal lethality, and less commonly to non-lethal motor weakness and developmental delays. Real-time Polymerase Chain Reaction:Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis. Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Article Title: Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients Article Snippet: The integrity of the extracted RNA was confirmed with Bioanalyzer (Agilent, Palo Alto, CA, USA). .. Reverse transcription was performed using 300 ng of total RNA with a TaqMan reverse transcription kit (Applied Biosystems, Perkin-Elmer Corporation, CA, USA). cDNA was amplified using specific primers together with probes predesigned by Applied Biosystems for organic transporter cation 1 (OTC1; cat. no. Hs00222691_m1), OTC2 (cat. no. Hs01010726_m1), OTC3 (cat. no. Hs00427552_m1), choline acetyltransferase (ChAT; cat. no. Hs00252848_m1), high-affinity choline transporter (ChT1; cat. no. Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL-22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Amplification:Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis. Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Article Title: Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients Article Snippet: The integrity of the extracted RNA was confirmed with Bioanalyzer (Agilent, Palo Alto, CA, USA). .. Reverse transcription was performed using 300 ng of total RNA with a TaqMan reverse transcription kit (Applied Biosystems, Perkin-Elmer Corporation, CA, USA). cDNA was amplified using specific primers together with probes predesigned by Applied Biosystems for organic transporter cation 1 (OTC1; cat. no. Hs00222691_m1), OTC2 (cat. no. Hs01010726_m1), OTC3 (cat. no. Hs00427552_m1), choline acetyltransferase (ChAT; cat. no. Hs00252848_m1), high-affinity choline transporter (ChT1; cat. no. Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL-22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Expressing:Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis. Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL-22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Control:Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis. Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL-22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Gene Expression:Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis. Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Article Title: Functional expression of choline transporter like-protein 1 (CTL1) and CTL2 in human brain microvascular endothelial cells. Article Snippet: In this study, we examined the molecular and functional characterization of choline transporter in human brain microvascular endothelial cells (hBMECs).. Choline uptake into hBMECs was a saturable process that was mediated by a Naþ-independent, membrane potential and pH-dependent transport system.. The cells have two different [3H]choline transport systems with Km values of 35.0 ± 4.9 mM and 54.1 ± 8.1 mM, respectively. Article Title: Protein kinase C promotes choline transporter-like protein 1 function via improved cell surface expression in immortalized human hepatic cells Article Snippet: Total RNA was extracted from the cells using a QIAshredder and RNeasy Mini kit (Qiagen Inc.) according to the manufacturer's instructions. .. TaqMan probes for Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL-22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Polymerase Chain Reaction:Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis. Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Article Title: Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis Article Snippet: Genomic DNA was eliminated by treatment with DNase 1 (Qiagen), and 100 ng purified RNA was reverse transcribed into first-strand cDNA using the CellScript cDNA Synthesis Master Mix (CellSafe, Suwon, Korea), which includes a genomic DNA elimination step. .. Real-time PCR amplification and relative expression quantification of IL-12, IL-2, IFN-γ, IL-23, IL-17A, IL-22, and GAPDH as a control were performed by TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA; Assay ID: CHT1, Reverse Transcription:Article Title: Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients Article Snippet: The integrity of the extracted RNA was confirmed with Bioanalyzer (Agilent, Palo Alto, CA, USA). .. Reverse transcription was performed using 300 ng of total RNA with a TaqMan reverse transcription kit (Applied Biosystems, Perkin-Elmer Corporation, CA, USA). cDNA was amplified using specific primers together with probes predesigned by Applied Biosystems for organic transporter cation 1 (OTC1; cat. no. Hs00222691_m1), OTC2 (cat. no. Hs01010726_m1), OTC3 (cat. no. Hs00427552_m1), choline acetyltransferase (ChAT; cat. no. Hs00252848_m1), high-affinity choline transporter (ChT1; cat. no. Migration:Article Title: Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients Article Snippet: The integrity of the extracted RNA was confirmed with Bioanalyzer (Agilent, Palo Alto, CA, USA). .. Reverse transcription was performed using 300 ng of total RNA with a TaqMan reverse transcription kit (Applied Biosystems, Perkin-Elmer Corporation, CA, USA). cDNA was amplified using specific primers together with probes predesigned by Applied Biosystems for organic transporter cation 1 (OTC1; cat. no. Hs00222691_m1), OTC2 (cat. no. Hs01010726_m1), OTC3 (cat. no. Hs00427552_m1), choline acetyltransferase (ChAT; cat. no. Hs00252848_m1), high-affinity choline transporter (ChT1; cat. no. Sequencing:Article Title: Functional expression of choline transporter like-protein 1 (CTL1) and CTL2 in human brain microvascular endothelial cells. Article Snippet: In this study, we examined the molecular and functional characterization of choline transporter in human brain microvascular endothelial cells (hBMECs).. Choline uptake into hBMECs was a saturable process that was mediated by a Naþ-independent, membrane potential and pH-dependent transport system.. The cells have two different [3H]choline transport systems with Km values of 35.0 ± 4.9 mM and 54.1 ± 8.1 mM, respectively. Article Title: Protein kinase C promotes choline transporter-like protein 1 function via improved cell surface expression in immortalized human hepatic cells Article Snippet: Total RNA was extracted from the cells using a QIAshredder and RNeasy Mini kit (Qiagen Inc.) according to the manufacturer's instructions. .. TaqMan probes for |
![Fig. 1 Radioactive choline uptake assay of human <t>CHT1</t> expressed in HEK293 cells. a Radioactive choline uptake of the CHT1-expressing HEK293 cells <t>(wild-type,</t> blue bar) as compared to the background radioactivity from the control cells transfected with the empty vector (EV, gray bar) 10 min after adding 0.1 mM choline with 10% of [3H]-choline. The inhibition of CHT1-mediated choline uptake was measured at 0.1 mM HC3 or 1 mM ML352. Data are mean ± SEM (n = 3 independent experiments). One-way ANOVA; ****P ≤0.0001. b Concentration- dependent choline uptake. Data points are mean ± SEM (n = 3 independent experiments) and fitted to the Michaelis–Menten equation with KM = 3.86 ± 0.68 mM. c Na+ and Cl–-dependent choline uptake. Data are mean ± SEM (n = 4 independent experiments) and are normalized against the radioactivity measurement with NaCl in the reaction solution. One-way ANOVA; ****P ≤0.0001. d, e Concentration-dependent inhibition of CHT1-mediated choline uptake by HC3 (d) and ML352 (e). Data points are mean ± SEM (n = 3 for HC3 and n = 3–6 for ML352) and fitted to the three-parameter dose–response curves (GraphPad Prism 9) with IC50 of 4.98 ± 1.04 nM for HC3 and 168.6 ± 49.4 nM for ML352.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_7078/pm39587078/pm39587078__page2_image1.jpg)
